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KMID : 0360220200610091015
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Journal of the Korean Ophthalmological Society
2020 Volume.61 No. 9 p.1015 ~ p.1022
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Ultraviolet B-induced Senescence Model Using Corneal Fibroblasts and the Anti-aging Effect of Angiogenin
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Sung Su-Min
Lee Soo-Jin
Lee Kyoo-Won
Kim Jae-Chan
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Abstract
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Purpose: To establish a corneal-fibroblast senescence model induced by ultraviolet B (UVB) exposure and to investigate the anti- senescence effect of angiogenin (ANG).
Methods: Fibroblasts were exposed to UVB (1 mJ/cm2) and then cultured with ANG-containing solution for 24 hours. The 24-hour culture procedure was repeated for three days after UVB irradiation. Cell viability was evaluated using the 3-[4, 5?dimethylthiazol- 2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay. The degree of senescence was assessed using the ratio of senescence- associated (SA)-β-gal-stained cells to total cells. The expression of age-related factors and degree of DNA damage were assessed via Western blot. Samples were divided into a non-UVB group, a UVB group without ANG treatment, and a UVB group with ANG treatment after irradiation (UVB + ANG).
Results: Cell viability in the UVB + ANG group was 11% higher than that in the UVB group (p < 0.05). The UVB + ANG group exhibited a 10% lower degree of SA-β-gal staining compared with the UVB group (p < 0.05). Western blot analysis showed that there was reduced expression of p53, p21, p16, and RB in the UVB + ANG group compared with the UVB group. The expression of phosphorylated histone (γ-H2AX) and p38 in the UVB + ANG group was less than that in the UVB group.
Conclusions: Senescence in corneal fibroblasts is induced by UVB, and ANG may exert an anti-aging effect by regulating the cell cycle through p53, p21, p16, and RB and reducing DNA damage.
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KEYWORD
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Angiogenin, Fibroblast, Senescence
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